polynucleotide phosphorylase การใช้

• The most ancient polyadenylating enzyme is polynucleotide phosphorylase.
• Like in bacteria, polyadenylation by polynucleotide phosphorylase promotes degradation of the RNA in plastids and likely also archaea.
• While many bacteria and mitochondria have polyadenylate polymerases, they also have another type of polyadenylation, performed by polynucleotide phosphorylase itself.
• In 1955, Marianne Grunberg-Manago and colleagues published a paper describing the enzyme polynucleotide phosphorylase, which cleaved a phosphate group from nucleotide diphosphates to catalyze their polymerization.
• The RNA element's function is likely analogous to an RNA structure found upstream of polynucleotide phosphorylase genes in " E . coli " and related enterobacteria.
• The structure of the S1 RNA-binding domain from the " Escherichia coli " polynucleotide phosphorylase has been determined using NMR methods and consists of a five-stranded antiparallel beta barrel.